 | Patrick Foy Esq. | 1850 - 1858 |
 | Rev. Bro. John Joseph Brown OMI | 1859 - 1861 |
 | Rev. Bro. Patrick Joseph Conway OMI | 1862 - 1870 |
| Rev. Bro. Michael A. Murphy OMI | 1870 | |
 | Rev. Bro. ( Fr.) Patrick O Flanagan OMI | 1870 - 1874 |
| T.W. Mc Mahan Esq | 1874 - 1875 | |
| Rev. Fr. Patrick O Flanagan OMI | 1875 - 1880 |
 | Rev. Bro. (Fr.) J.A.R. Smythe OMI | 1880 - 1883 |
 | Rev. Fr. Charles H. Lytton OMI | 1883 - 1885 |
 | Rev. Fr. Jules Collin OMI | 1885 - 1888 |
 | Rev. Fr. M. Dubreuil OMI | 1888 - 1889 |
 | Rev. Fr. Patrick Dunne OMI | 1889 - 1901 |
| Rev. Fr. Jules Collin OMI | 1901 - 1902 |
 | Rev. Fr. Charles A. Beaud OMI | 1902 - 1905 |
 | Rev. Fr. Charles S. Matthews OMI | 1905 - 1921 |
 | Rev. Fr. John A. Guyomar OMI Consecrated as Bishop of Jaffna in 1924 | 1921 - 1924 |
| Rev. Fr. Charles S. Matthews OMI | 1924 - 1936 |
 | Rev. Fr. Timothy M.F. Long OMI | 1936 - 1954 |
 | Rev. Fr. S.N. Arulnesan OMI | 1954 - 1960 |
 | Rev. Fr. P.J. Jeevaratnam OMI | 1960 - 1966 |
 | Rev. Fr. T.A.J. Mathuranayagam | 1966 - 1976 |
 | Rev. Fr. John A. Francis OMI | 1976 - 1979 |
 | Rev. Fr. G.A. Michael (Francis) Joseph | 1979 - 1992 |
 | Rev. Fr. A.I. Bernard | 1992 - 2002 |
 | Rev. Fr. Dr. Justin Gnanapragasam Consecrated as Bishop of Jaffna on 28 November 2015 | 2002 - 2007 |
 | Rev. Fr. Jero Selvanayagam | 2008 - Current |
